Improved surface culturing of Mycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
Improved surface culturing of Mycobacterium tuberculosis.
In studies on surface components of virulent mycobacteria, there is a continuous need for relatively large quantities of these organisms as produced in surface culture in Roux bottles. Achieving effective surface growth depends upon adequately seeding the surface of the liquid medium. A practice which may well have been employed even by Koch has been the transfer of a pellicle of "veil growth" ...
متن کاملMagnetic bead protocol for culturing Mycobacterium tuberculosis from sputum specimens.
A magnetic bead protocol and a standard centrifugation protocol yielded Mycobacterium tuberculosis in 40/50 sputum specimens in 12 ± 1 days and 11 ± 2 days, respectively (P > 0.05 by Student's t test). Manipulation took 35 ± 5 min and 45 ± 10 min, respectively (P < 0.05). The magnetic bead protocol could advantageously replace centrifugation for culturing M. tuberculosis from sputum.
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Tuberculosis remains a major public health problem in both developing and developed countries. Drug-resistant tuberculosis is an increasing health problem and serious challenge to tuberculosis (TB) control programs. Information about the susceptibility pattern of Mycobacterium tuberculosis isolates against anti-tuberculosis drugs is an important aspect to TB control. The objectives of the s...
متن کاملEvaluation of the Kudoh swab method for the culturing of Mycobacterium tuberculosis in rural areas.
OBJECTIVE To compare the simple, swab 'Kudoh method' for culturing Myobacterium tuberculosis from sputum samples, to the standard Petroff digestion-decontamination procedure. The Kudoh method, which requires no centrifugation and takes only 4-5 min per sample, was also evaluated for its performance in a rural setting. METHODS Two hospital laboratories in Caracas, Venezuela processed 314 sputu...
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The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isola...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1967
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.94.4.1258-1259.1967